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Purposeful validation of sorted fractions was executed by xenotransplantation, the results of which was combined with miRNA expression profiling to produce stem mobile-linked miRNA expression profiles.
To find out whether miRNA are differentially expressed in LSC and HSC, we fractionated sixteen AML individual samples and a few lineage-depleted (Lin–) cord blood (CB) samples working with CD34 and CD38 into four populations and subjected Each individual to world-wide miRNA profiling; the stem mobile content material of every portion was functionally assayed by xenotransplantation (Figures 1A and S1A).
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(J) Proliferation calculated by BrdU incorporation assay of CTRL or 126KD transduced 8227 cells in vitro.
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The LSC frequency was diminished in two of 3 samples upon 126KD (Figures 5C and S5D). With each other, these findings recommend that 126KD produces heterogeneous responses with LSC function and frequency decreased inside a subset of AML patients.
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(D) Consultant intracellular movement plots for the detection of CDK3 and pRB Ser807/811. Graph down below signifies three impartial intracellular movement experiments for every issue exactly where the mean fluorescence intensity was compared. Signify ± SEM; ∗p=
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